Virtual Slide List

Resources

Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources.

Resources on M+Box

This collection was originally compiled by Kent Christensen, Ph.D., J. Matthew Velkey, Ph.D., Lloyd M. Stoolman, M.D., Laura Hessler, and Diedra Mosley-Brower. Currently, it is curated by Michael Hortsch, Ph.D. If you have questions or comments regarding the University of Michigan virtual slide collection, please contact Dr. Hortsch at hortsch at umich.edu.

Virtual Slide List for Histology Course

Search Description
Digestive System 1: Oral Cavity and Salivary Glands
185
Taste Bud: Note the difference in cell shape and cell aggregation of the taste bud and the oral epithelium. The nerve endings are sensory nerves. Be able to recognize a taste bud as such, but you will not be required to know its detailed structure and recognize different cell types.
187
Mucous Acinus of a Lingual Salivary Gland: Note the accumulation of mucin-containing secretory vesicles next to the lumen of the secretory acinus depicted in this electron micrograph.
Digestive System 2: Pharynx, Esophagus, Stomach
126
Trachea & esophagus, cross section, H&E, 40X
152
Oral pharynx, H&E, 40X (stratified squamous non keratinized epithelium, lamina propria, elastic fibers, muscularis, mucous glands).
152AF
Oral pharynx, Masson trichrome stain, 40X (stratified squamous non keratinized epithelium, lamina propria, elastic fibers, muscularis, mucous glands).
153
Esophagus, H&E, 20X (stratified squamous non keratinized epithelium, lamina propria, muscularis mucosae, submucosa, submucosal mucous glands, muscularis externa [all smooth muscle], myenteric [or Auerbach's] plexus).
155
Esophagus and stomach, H&E, 40X (ESOPHAGUS [at right]: epithelium, lamina propria, muscularis mucosae, submucosa, muscularis externa [all smooth muscle], myenteric [or Auerbach's] plexus; CARDIAC STOMACH [next to esophagus]: pits, pyloric glands, muscularis mucosae; FUNDIC STOMACH [at left]: pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis).
156
Stomach, H&E, 20X ( pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis).
156
Stomach, H&E, 40X ( pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis).
157
Stomach, H&E, 20X ( pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis).
157
Stomach, H&E, 40X ( pits, glands, mucous cells, parietal cells [acid], chief cells [pepsinogen], muscularis mucosae, submucosa, muscularis).
160
Gastro-esophageal junction, PAS & Azure Blue stain, 40X
162
Pyloric stomach [at left], duodenum of small intestine [at right], H&E, 20X (pyloric pits and glands).
162
Pyloric stomach [at left], duodenum of small intestine [at right], H&E, 40X.
190
Stratified Squamous Non-keratinized Epithelium (Esophagus): Note the characteristic stratified squamous non-keratinized epithelial lining of the esophagus.
197
Gastric Pits and Glands (Fundus): Note that there is only one cell type, a mucous cell, in the surface epithelium of the stomach. The gastric pits lead into the gastric glands proper, where there are several cell types present. As always, the lamina propria consists of loose connective tissue. There is a rich capillary network in the lamina propria.
198
Mucous Surface Cell (Stomach Fundus): Mucous granules accumulate in the apical cytoplasm before they are released.
203
Chief Cell (Stomach Fundus): Review the structure of the chief cell. It is essentially that of an exocrine gland cell - as seen in the salivary glands and the pancreas.
204
Parietal Cell (Stomach Fundus): Note the abundant vesicles and tubules of the parietal cell, which are involved in HCl production. Note the numerous giant mitochondria in the parietal cell as opposed to the small and sparse mitochondria in the chief cells. Study the intracellular secretory canaliculi of the parietal cell.
205
Enteroendocrine Cell (Stomach): Note the accumulation of secretory vesicles in this enteroendocrine cell. They are usually found adjacent to the basal and lateral sides of the cell, not the apical aspect, which is facing the lumen of the gastrointestinal tract. You will not be asked to discriminate between different subtypes of enteroendocrine cells.
Digestive System 3: Small and Large Intestine
029
Small intestine, H&E, 40X
161
Pylorus, duodenum, pancreas, H&E, 40X
162
Small intestine, duodenum [right side of section], H&E, 40X (villi very poorly preserved, glands [crypts of Lieberkühn], muscularis mucosae, submucosal glands).
165
Ileum, PAS stain, 40X
168
Small intestine, jejunum or ileum, H&E, 40X (plicae, villi, epithelium, goblet cells, lamina propria, intestinal glands [crypts of Lieberkühn], Paneth cells, muscularis mucosae, submucosa, muscularis externa, myenteric plexus), serosa).
169
Small intestine, jejunum or ileum, H&E, 20X (plicae, villi, epithelium, goblet cells, lamina propria, intestinal glands [crypts of Lieberkühn], Paneth cells, muscularis mucosae, submucosa, muscularis externa, myenteric plexus), serosa).
169
Small intestine, jejunum or ileum, H&E, 40X (plicae, villi, epithelium, goblet cells, lamina propria, intestinal glands [crypts of Lieberkühn], Paneth cells, muscularis mucosae, submucosa, muscularis externa, myenteric plexus), serosa).
170
Small intestine, ileum, H&E, 20X (contains nice Paneth cells).
170
Small intestine, ileum, H&E, 40X.
171
Small intestine, H&E, blood vessels injected with dye, 20X (observe small blood vessel distribution in villi and elsewhere).
175
Appendix, H&E, 40X (mucosa as in colon, prominent lymphoid nodules in submucosa).
176
Large intestine (colon), H&E, 40X (straight glands [crypts of Lieberkühn], no villi, simple columnar epithelium, goblet cells, lamina propria, muscularis mucosae, submucosa, muscularis externa, taenia coli).
177
Recto-anal junction, monkey, H&E, 40X
177-2
Rectoanal junction [anal canal at right], Masson trichrome, 20X (RECTUM: simple columnar epithelium in glands. ANAL CANAL: initially stratified columnar/cuboidal, then stratified squamous epithelium first non keratinized and then keratinized [thin skin, note hair follicles, sebaceous glands], no muscularis mucosae, veins, anal sphincter).
177-3
Recto anal junction [rectum at far right], H&E, 20X (RECTUM: simple columnar epithelium in glands. ANAL CANAL: initially stratified columnar/cuboidal, then stratified squamous epithelium first non keratinized and then keratinized [thin skin], no muscularis mucosae, veins, anal sphincter).
UCSF 246
Jejunum, human, H&E stain, 40X
UCSF 247
Jejunum, human, H&E and silver stain, 40X
207
Small intestine (Muscularis Externa): Study the orientation of the smooth muscle cells in the intestinal muscularis externa. The micrograph will help you understand the pattern, which arises from the inner circular layer and outer longitudinal layer of smooth muscle cells. Without the knowledge in which direction the intestinal epithelium is located, it is not possible to discriminate between the two sublayers of the muscularis externa.
208
Small Intestine (Tip of Villus, longitudinal section): The villus is covered by a simple columnar epithelium. Note that cells are sloughing off at the tip of the villus. Find some goblet cells, which represent one resident cell type of the intestinal epithelium. The small lymphocyte is transient and is not a permanent component of the epithelium. Find the “striated border” and realize that you can’t resolve individual microvilli at this magnification. Study the composition of the connective tissue core of the villus.
211
Small intestine (Base of a Villus in the Jejunum): This micrograph depicts the typical appearance of the epithelium at the base of an intestinal villus. Note the striated or brush border lining at the apical aspect of the simple columnar epithelium and the presence of mucous-secreting cells.
213
Microvilli: This micrograph shows the typical appearance of microvilli on the apical surface of a cell, which contributes to the striated or brush border lining in the in small intestine.
214
Crypts of Lieberkühn (Jejunum, cross section): In this simple tubular gland, Paneth cells are found near the end, whereas mucous cells and undifferentiated cells take up the major portion of the gland. Review the reasons for the high rate of cell mitosis in the upper part of the intestinal gland.
216
Large Intestine (Colon): Goblet cells are particularly numerous in the large intestine. If you look closely at the labeled goblet cell, you can see that the apex is packed with mucus-containing secretory vesicles. A single crypt is seen in the section and its lumen doesn’t stay in the plane of section all the way to the base.
Digestive System 4: Liver and Pancreas
1
Liver, H&E, 40X (central vein, portal triad [portal vein, bile duct, hepatic artery], hepatocyte, sinus, endothelium, Kupffer cell, space of Disse, liver lobule).
188
Pancreas, H&E, 20X (exocrine pancreas, lobule, acini, centroacinar cell, intercalated duct, endocrine pancreas [islets of Langerhans]).
188B
Pancreas, H&E, 40X
189
Pancreas, vascular injection, 40X (vascular pattern, high vascularity of islets).
190B
Pancreas, chrome alum hematoxylin and phloxine stain, differential staining of cells in islets of Langerhans, 40X (in an islet note that alpha cells [glucagon] have reddish cytoplasm, and beta cells [insulin] have pale blue green cytoplasm; delta cells [somatostatin] can't be distinguished).
194
Liver and gall bladder, H&E, 40X (LIVER: central vein, portal triad [portal vein, bile duct, hepatic artery], hepatocyte, sinus, endothelium, Kupffer cell, space of Disse, liver lobule. GALL BLADDER: epithelium, lamina propria, muscularis).
195
Liver and gall bladder, Masson trichrome, 40X (LIVER: distribution of collagen. GALL BLADDER: epithelium, lamina propria, muscularis ).

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