This collection was originally compiled by Kent Christensen, Ph.D., J. Matthew Velkey, Ph.D., Lloyd M. Stoolman, M.D., Laura Hessler, and Diedra Mosley-Brower. Currently, it is curated by Michael Hortsch, Ph.D. If you have questions or comments regarding the University of Michigan virtual slide collection, please contact Dr. Hortsch at hortsch at umich.edu.
Virtual Slide List
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Resources
Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources.
Virtual Slide List for Histology Course
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106
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Thick skin, sole of foot, H&E, 40X (stratified squamous epithelium, keratinized). | |
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112
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Thick skin, sole of foot, H&E, 40X (stratified squamous keratinized epithelium) | |
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126
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Trachea & esophagus, cross section, H&E, 40X (Trachea: pseudostratified/respiratory epithelium; esophagus: stratified squamous nonkeratinized epithelium) | |
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153
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Esophagus, H&E, 20X (stratified squamous epithelium, nonkeratinized). | |
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176
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Colon, H&E, 40X (simple columnar epithelium). | |
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210
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Kidney, H&E, 40X (simple cuboidal epithelium). | |
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211
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Non-distended ureter, human, H&E, 40X (transitional epithelium) | |
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250-1
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Vagina, H&E, 40X (stratified squamous epithelium, nonkeratinized). | |
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Duke University 98
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Non-distended bladder, H&E, 40X (transitional epithelium) | |
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016
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Desmosome and Intermediate Filaments: Look also at the structure and appearance of other cell junctions in electron micrographs. This image depicts a typical desmosomal junction in the upper right corner of the image. Note its association with abundant intermediate filaments on the cytoplasmic side of the plasma membrane. | |
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065
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Stratified Squamous Keratinizing Epithelium: You can appreciate that this epithelium (skin) is stratified (has multiple layers of cells) and that the layers near the surface (at the top of the micrograph) have keratinized (lost their nuclei and become a layer of keratin). The spiny appearance of cells deeper in the epithelium can also often be seen in the light microscope. They are points of cell-cell attachment, made more obvious by shrinkage during preparation. | |
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120
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Simple Squamous Epithelium: The endocardium, the simple squamous epithelial lining of the heart, can be seen at the top of this section. Under it, you can see connective tissue, which we will study next time. Note how thin the epithelium is. | |
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153
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Pseudostratified Epithelium: The definition of a pseudostratified epithelium is one in which there are multiple levels of nuclei, but all cells extend to the base of the epithelium. In this micrograph of the epithelial lining of a trachea, you can find cells with nuclei at different levels which can be traced down until they are at least close to the base of the epithelium before some of them go out of the plane of section. Note also the apical cilia and basal bodies. You can see that the goblet cells are not ciliated and are polarized for secretion, i.e., the nucleus is basal to the clear secretion granules, which will be released into the lumen at the top of the micrograph. | |
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154
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Respiratory Epithelium (tangential section): The yellow colored area indicates the outline of one cell (see previous wall chart #153 for plane of section). Note the tops of the goblet cells protruding between the cilia. | |
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156
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Cilia: Cross sections of cilia. The typical 9+2 arrangement of the microtubules is especially evident. | |
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170
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Simple Cuboidal Epithelium with Brush Border: The micrograph depicts epithelial lining cells of a proximal tubule in the kidney. The typical appearance of microvilli on the apical surface of simple cuboidal epithelial cells with a striated or brush border is seen in this micrograph. | |
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183
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Transitional Epithelium: This micrograph displays the transitional epithelial lining of the bladder. | |
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190
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Stratified Squamous Non-keratinizing Epithelium: Compare this micrograph to the previous one. This is the lining of the esophagus, where it is no longer necessary to have an outer keratinized layer to protect against desiccation, as it was for skin. Thus, the outermost layer is still cellular and contains a nucleus. Note again the spiny appearance of the cells, due to the desmosomal attachments. | |
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211
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Simple Columnar Epithelium: You can see that this type of epithelium, which is lining the lumen of the jejunum of the small intestine, is a simple epithelium: It is only one cell layer thick and columnar, as the cells are rather tall. Note the basal lamina at the base of the epithelium. In some places you can see the apical area where junctions are located. Most of these cells also have short apical microvilli. | |
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213
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Microvilli: This micrograph shows the typical appearance of microvilli on the apical surface of a cell, which contributes to the striated or brush border lining in the in small intestine. |
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EYE-1
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Posterior eye (retina), H&E, 20X | |
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EYE-2
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Eyeball, H&E, 20X | |
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UCSF 164
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Eye, Masson stain, 20X | |
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UCSF 173
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Eyelid, H&E, | |
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322
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Human Cornea: Know and recognize the different cellular and acellular layers of the cornea. | |
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326
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Human Iris | |
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330
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Human Ciliary Process Epithelium: Note the different appearances of the two epithelial layers of the non-visual part of the retina. | |
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331
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Human Retina: Know the different layers of the visual part of the retina, specifically the location of the different types of neuronal cells and where they connect with each other. |
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234-1
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Ovary, H&E, 40X (other examples, corpus luteum). | |
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234-2
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Ovary, Masson trichrome stain, 20X (other examples, corpus luteum]). | |
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235
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Ovary, H&E, 20X (other examples). | |
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236a
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Ovary, H&E, 40X (other examples, corpus luteum, degenerating corpus luteum [almost a corpus albicans). | |
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239
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Ovary, monkey, H&E, 40X (hilus, mesovarium, medulla, cortex, tunica albuginea, primordial follicles, ovum, granulosa [follicular] cells, primary follicles, secondary follicles, antrum, mature [Graafian] follicles,theca interna, zona pellucida, cumulus oophorus, corona radiata, atresia, glassy membrane, corpus luteum, granulosa lutein cells, theca lutein cells, corpus albicans). | |
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240-1
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Oviduct, H&E, 40X (infundibulum, fimbriae). | |
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240-2
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Oviduct, H&E, 40X (ampulla, ciliated cells, peg cells). | |
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241even
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Oviduct, H&E, 40X (isthmus [at left]). | |
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241odd
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Oviduct, uterine segment, H&E, 40X | |
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269
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Ovary, monkey, PAS, 40X | |
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269-2
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Ovary, monkey, PAS, 40X | |
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279
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Primordial Follicle: Note an incomplete layer of flat granulose cells around the oocyte. | |
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280
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Primary Oocyte: Note the highly organized appearance of the primary follicle and the considerable size difference to a primordial follicle. | |
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281
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Secondary Follicle: In this low magnification view of a secondary follicle, examine the characteristics of the thecal layer, granulosa cells, corona radiata and the ooplasm. Note particularly the basal lamina which separates the thecal and granulosa cell layers and the acellular zona pellucida that surrounds the oocyte. | |
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282
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Zona Pellucida of a Secondary Follicle: Higher magnification view of another secondary follicle. Note the penetration of the zona pellucida by processes from the oocyte and from the granulosa cells of the corona radiata. | |
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283
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Theca Interna and Externa of a Secondary Follicle: The cells of the theca interna will eventually differentiate into endocrine cells and show characteristics of steroid-producing cells. The theca externa consists of a mixture of smooth muscle cells and connective tissue. | |
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285
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Corpus Luteum: You do not need to be able to identify theca and granulose lutein cells based on their EM appearances. However, note the difference in their cytoplasmic contents and know their secretory products. | |
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288
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Epithelium of the Oviduct: Cilia and their basal bodies are clearly visible in the apical cytoplasm of the ciliated cells. Note short microvilli on the non-ciliated secretory cells. |
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243
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Uterus, stage between proliferative and secretory, H&E, 20X (mucosa = endometrium [at right], glands, coiled or spiral arteries, myometrium [middle], smooth muscle, perimetrium = connective tissue and serosa [peritoneum] [at left]). | |
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244
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Uterus, proliferative stage, H&E, 40X (another example, coiled or spiral arteries). | |
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245-1
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Uterus, secretory stage, H&E, 40X (endometrium with secretory glands, coiled or spiral arteries). | |
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245-2
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Uterus, menstrual stage, H&E 40X (stratum functionalis sloughing off, heavy lymphocytic infiltration). |