This collection was originally compiled by Kent Christensen, Ph.D., J. Matthew Velkey, Ph.D., Lloyd M. Stoolman, M.D., Laura Hessler, and Diedra Mosley-Brower. Currently, it is curated by Michael Hortsch, Ph.D. If you have questions or comments regarding the University of Michigan virtual slide collection, please contact Dr. Hortsch at hortsch at umich.edu.
Virtual Slide List
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Resources
Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources.
Virtual Slide List for Histology Course
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249
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Cervix, H&E, 20X (cervical glands [below] with columnar epithelium [secretes mucus], muscle [above], uterine wall [at left], vagina [at right]). | |
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250-1
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Vagina, H&E, 40X (stratified squamous epithelium non keratinized, connective tissue, smooth muscle [inner circular, outer longitudinal]). | |
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250-2
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Vagina, Masson trichrome stain, 40X (another example, distribution of connective tissue [blue] and smooth muscle [reddish] seen particularly well, many nerves). | |
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UCSF 405
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Cervix, trichrome stain, 40X |
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253
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Placenta, early, H&E, 40X (villi, maternal blood space, syncytiotrophoblast layer, cytotrophoblast layer, Hofbauer cells = fetal macrophages) | |
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255
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Placenta, late, H&E, 20X (villi). | |
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255
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Placenta, late, H&E, 40X (villi, maternal blood space, syncytiotrophoblast, occasional cytotrophoblast,capillary, macrophage [Hofbauer cell), fibrinoid). | |
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259
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Mammary gland, inactive = nulliparous, H&E, 20X (glandular lobules, ducts, myoepithelial cells, intralobular and interlobular connective tissue). | |
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258
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Mammary gland, active [perhaps middle of pregnancy], H&E, 40X (alveoli, myoepithelium, plasma cells, ducts, lactiferous ducts). | |
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261
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Mammary gland, active, lactating, H&E, 40X | |
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265
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Nipple and areola, H&E, 20X (nipple [center], areola [at left and right], lactiferous ducts, myoepithelial cells, thin skin, sebaceous glands, smooth muscle strands, nerves). | |
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289
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Placental Villus (mid pregnancy): Examine the ultrastructure of the syncytiotrophoblast cells, particularly their external surface. Note the paucity of cytotrophoblast cells at this stage of development. Be certain that you know the components of the placental barrier, how they may change during pregnancy and why. | |
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290
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Mammary Gland: Observe the process of true apocrine secretion. Lipid granules are released covered with a small amount of mammary gland cell cytoplasm, as well as the cell membrane. On the other hand, the protein components are released by the usual mechanism of exocytosis. |
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26
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Lymph node, H&E, 20X (lymph node at upper right). | |
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26
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Lymph node, H&E, 40X (detail of above, capsule, afferent lymphatic vessels, subcapsular sinus, cortex, follicles=nodules [mostly B cells], germinal centers [abnormal here, see slide 30], parafollicular cortex=deep cortex [mostly T cells], high endothelial venules, medulla, cords, sinuses, endothelial cells, reticular cells, hilus). | |
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27
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Lymph node, H&E, 40X (see also slide26 40X above for structures, germinal centers). | |
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28-2
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Lymph node, stained with silver to show reticular fibers [black], 40X (reticular fibers are made by reticular cells). | |
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30
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Lymph node [in mesentery see dark structures], H&E, 40X (see also slide 26 40X for follicular germinal centers, high endothelial venules). | |
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138
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Tonsil, palatine, H&E, 20 (crypt [white space beginning at top and extending down], crypt lined with stratified squamous epithelium non-keritanized, lymphatic follicles with large germinal centers). | |
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138
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Tonsil palatine, H&E, 40X | |
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138L
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Tonsil, faucial, H&E, 40X (several crypts lined with stratified squamous epithelium non keritanized, lymphoid follicles). | |
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140
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Thymus, young, H&E, 40X (cortex [darker, developing T cells], medulla [lighter], epithelial reticular cells, macrophages, Hassall's corpuscles). | |
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142
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Lymph node, H&E, 40X (see also slide 26 40X for germinal centers, high endothelial venules). | |
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147B
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Spleen, H&E, 40X (capsule, trabeculae [dense c.t., stains red], white pulp, central arterie w/periarteriolar lymphatic sheath = PALS [mostly T cells], lymphatic follicles [mostly B cells, sometimes with germinal center], marginal zone, red pulp, splenic cords, venous sinuses, reticular cells). | |
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148
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Spleen, H&E, 20X | |
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148
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Spleen, H&E, 40X (detail of previous slide, red pulp sinuses). | |
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141
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Thymus, adult, 40X (extensive fatty tissue, cortex and medulla quite involuted). | |
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157b
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Fundic stomach, H&E, 40X | |
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161
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Gastroduodenal junction, H&E, 40X | |
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168
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Small intestine, jejunum, H&E, 40X | |
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175
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Appendix, H&E, 40X | |
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301
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Spleen, H&E, 40X (see slide 147 20X above for central artery with PALS and lymphatic follicles with large germinal centers). | |
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UCSF 261
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Appendix, H&E, 40X | |
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294
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Lymph Node (Subcapsular Sinus and Cortex): Find the subcapsular sinus and the reticular cells of the node. | |
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296
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Medullary Cord (Lymph Node): Blood vessels are restricted to the medullary cords. Plasma cells occur abundantly in the cords. Review the function and origin of plasma cells. Note that the free, circulating lymphocytes are either small or medium size. | |
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297
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Postcapillary High Endothelial Venule (Lymph Node): Note the unusual height of the lining endothelial cells and the lymphocytes of different sizes in transit from the vessel into the lymph node. A small fraction of lymphocytes enter the node through afferent lymphatic vessels. | |
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298
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Medullary Sinus (Lymph Node): Observe the loose arrangement of lymphoid tissue in the medulla of lymph nodes. The sinuses interconnect and their lumen is traversed by reticular cells, some of which can trap antigens and some of which are true phagocytes. In addition, reticular cells wrap around the bundles of reticular fibrils and thereby isolate them from lymphocyte access. | |
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303
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White and Red Pulp (Spleen): Note the very dense arrangement of lymphoid cells in the white pulp and the loose arrangement of reticular cells, some lymphoid cells and extravasated erythrocytes in the pulp cords between the venous sinuses. Note also the marginal zone and recall that this is where most antigen presentation occurs in the spleen. | |
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306
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Marginal Zone (Spleen): The marginal zone is a cell rich region at the interphase between white and red pulp. As it is the region of antigen presentation, a large number of macrophages and different types of lymphocytes reside here. | |
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310
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Red Pulp with Venous Sinus (Spleen): Find the venous sinuses and observe the movement of erythrocytes across the wall. The presence of many macrophages in the interstitial space of the red pulp is obvious. | |
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318
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Thymus (Cortex and Medulla): Note that the thymus does not have lymph nodules. However, small lymphocytes (thymocytes) are aggregated in the cortex and larger, epithelial reticular cells can be identified in the center (medulla), giving the appearance of a “nodular” area. | |
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319
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Thymus (Medulla): Remember that Hassall’s corpuscles (or bodies) are found only in the thymus. | |
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321
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Blood-Thymus Barrier: Review the components of the blood-thymus barrier. |
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075
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Seminal vesicle, H&E, 20X | |
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270
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Testis, human, H&E, 40X (testis [upper left], seminiferous tubules, spermatogonia, primary spermatocytes, secondary spermatocytes [very seldom seen], spermatids, Sertoli cells, interstitial tissue, Leydig cells, small blood vessels, tunica albuginea, mediastinum [upper right], rete testis, epididymis [lower half of section], efferent ducts). | |
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270ex
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Testis, H&E, 40X | |
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273
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Testis, immature human, H&E, 40X (testis, seminiferous tubules with only early germ cells and poorly developed Sertoli cells, small Leydig cells). | |
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275
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Testis, human, H&E, 40X (testis [lower half of section], seminiferous tubules, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, interstitial tissue, Leydig cells, small blood vessels, tunica albuginea, epididymis [upper right], efferent ducts). | |
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279
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Seminal vesicle, human, H&E, 40X (three lumens of contorted tube, complex interconnecting mucosal folds, secretory epithelium [simple columnar or pseudostratified columnar], lamina propria, abundant smooth muscle in wall of tube). | |
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281
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Prostate, human, H&E, 40X (prostatic urethra [at center], numerous prostatic glands [left, right and middle], secretory epithelium [simple or pseudostratified columnar], abundant smooth muscle in the connective tissue between the glands, two ejaculatory ducts with the prostatic utricle = utriculus masculinus between them. |