Virtual Slide List


Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources.

Resources on M+Box

This collection was originally compiled by Kent Christensen, Ph.D., J. Matthew Velkey, Ph.D., Lloyd M. Stoolman, M.D., Laura Hessler, and Diedra Mosley-Brower. Currently, it is curated by Michael Hortsch, Ph.D. If you have questions or comments regarding the University of Michigan virtual slide collection, please contact Dr. Hortsch at hortsch at

Virtual Slide List for Histology Course

Search Description
Male Reproductive System
Prostate gland, Masson stain, 20X
Prostate, human, H&E, 20X (prostatic glands, with abundant smooth muscle between them, concretions [= amyloid bodies] in many of the glands).
Spermatic cord, human, H&E, 20X (ductus or vas deferens [top of section], branch of testicular artery [center of section], pampiniform plexus of testicular veins [around testicular artery branches], dartos muscle [smooth], cremaster muscle [skeletal].
Penis cross section, human, H&E, 40X (two large pale corpora cavernosa [erectile tissue], venous sinuses, trabeculae [connective tissue, smooth muscle, small blood vessels including helicine arteries], deep artery, tunica albuginea, penile urethra [at lower right] in corpus spongiosum).
UCSF 363
Testis, human, Regaud-Hem & van Gieson stain, 40X
Seminiferous Tubule: In this electron micrograph, showing a seminiferous tubule and adjacent interstitial tissue, you can see in more detail the same structures you have been viewing with the light microscope. In the seminiferous tubule, the large, round nuclei belong to more mature primary spermatocytes. Toward the lumen from these are the nuclei of spermatids. Note the occasional Sertoli cell nuclei and try to follow the extensive Sertoli cell cytoplasm as it passes up to enfold the spermatocytes and spermatids. In the interstitial tissue, observe the Leydig cells and capillaries (the latter are somewhat swollen because the testis was fixed for electron microscopy by perfusion of the fixative through the blood vessels).
Seminiferous Epithelium: This electron micrograph shows the seminiferous epithelium at a higher power than the previous electron micrograph (EM #258). Note the different appearances of the cell nuclei in spermatogonia, primary spermatocytes, Sertoli cells and spermatids.
Testis (Interstitial tissue): Clearly visible are a number of Leydig cells in the interstitial tissue of the testis: Also note a postcapillary venule, a capillary and lymphatic spaces.
Epididymis (Pseudostratified Epithelium): Tall cells of uniform height extend to the lumen, while short basal cells are limited to the base of the tubule. Note most of the stereocilia are transversely sectioned.
Small intestine, H&E, 40X (smooth muscle). See Epithelium/CT section.
Heart, ventricle, H&E, 40X (cardiac muscle, intercalated discs).
Skeletal muscle, longitudinal section, H&E, 20X and 40X (banding pattern A I Z H [60787 x 18392, 34840 x 9034, 64339 x 17968]).
Skeletal muscle, longitudinal section, H&E, 40X.
Skeletal muscle, cross section, H&E, 40X.
058 Thin section
Skeletal muscle, longitudinal section, H&E, 83X
Fetal forearm muscle, cross section, H&E, 40X (fascicle, epimysium, perimysium, endomysium).
Fetal forearm muscle, Masson, 40X (fascicle, epimysium, perimysium, endomysium).
Heart, ventricle, H&E, 40X (cardiac muscle, intercalated discs). See Cardiovascular section.
Heart wall, trichrome stain, 40X
gastro-esophageal junction H&E longitudinal, 40X (smooth muscle).
Small intestine, H&E, 40X (smooth muscle).
Vagina, H&E, 40X (stratified squamous epithelium, nonkeratinized).
Vagina, Masson trichrome stain, 40X (smooth muscle). See Female Reproduction section.
Heart, H&E, 40X (cardiac muscle, intercalated discs). See Cardiovascular section.
Skeletal Muscle (longitudinal section): Identify a sarcomere. Relate the sarcomeric structure seen in the LM to the structure seen here. Note that there is also lots of glycogen in the region between the two myofibrils in this picture, a storage form for glucose (which is metabolized to provide energy for muscle contraction). At the border of the I and A-bands, note triads consisting of a central T (transverse) tubule and flanking cisternae of the sarcoplasmic reticulum.
Skeletal Muscle (cross section, low magnification): Note location of muscle fiber nuclei. You can see cross sections of A bands (darker) and I bands (lighter) side by side in the same cell because of the fact that the myofibrils don't line up perfectly. Identify the approximate outline of a single myofibril.
Skeletal Muscle (longitudinal section, low magnification): Find the skeletal muscle nuclei and note their peripheral location. Note the intimate contact between capillaries and muscle cells and be sure you can tell where one muscle cell or fiber stops and another begins (you can see parts of four fibers in this picture). Make sure you know which is the longitudinal axis of the cell. Identify sarcomeres, A bands, I bands, Z lines and H zones. Note that, as you saw at the LM level, the individual myofibrils do not line up perfectly across the fiber.
Cardiac Muscle (Intercalated Disc, longitudinal section): Note the somewhat irregular course of the intercalated disc. In this preparation, the I bands are very short, indicating that the sarcomere is in a contracted state. Review the types of junctions present in an intercalated disc and their functions.
Cardiac Muscle (longitudinal section): Note central location of muscle nuclei. Note the "stacks" of mitochondria between myofibrils. Cardiac muscle is even richer than skeletal muscle in mitochondria (again, important for energy production). An intercalated disc is present in the upper left region of the picture.
Smooth Muscle (cross section): Here you can see the filaments in cross-section, appearing as dots. Also, the dark areas, which are membrane-associated, are called dense plaques and are sites of filament attachment.
Smooth Muscle (longitudinal and cross section): Study the orientation of the smooth muscle layers in the intestine and their appearance. The micrograph will help you understand the patterns, which arise from longitudinal and cross-sections of smooth muscle.
Peripheral Nervous Tissue
Small intestine, H&E, 40X (with myenteric plexi between the two layers of the muscular externa).
Spinal cord, cross section, Masson, 40X (spinal cord, neuron, dorsal root ganglion).
Spinal cord, cross section, H&E, 40X (spinal cord, neuron, dorsal root ganglion).
Spinal cord, cross section, Masson, 40X (spinal cord, neuron, dorsal root ganglion).
Thoracic spinal cord, luxol blue stain, 40X
Peripheral nerve, longitudinal section, H&E, 40X (axon, myelin, nodes of Ranvier).
Peripheral myelinated nerve, cross section, 40X (fascicle, axon, endoneurium, perineurium, epineurium).
Muscle and muscle spindle, trans. section, H&E, 40X
Muscle and muscle spindle, trans. section, H&E, 40X
Muscle fibers, Golgi colloidal gold stain, 20X (with motor end plate = neuromuscular junction).
Sympathetic ganglion, probably methylene blue stain, 40X.
Seminal vesicle, H&E, 20X (parasympathetic ganglion).
Seminal vesicle, H&E, 40X (with parasympathetic ganglion).
Gastro-esophageal junction, longitudinal section, H&E, 40X
Jejunum, cross section, H&E, 40X
Vagina, H&E, 40X (nerve fibers and parasympathetic ganglion).
Vagina, Masson trichrome stain, 40X (nerve fibers and parasympathetic ganglion)
Motor Neuron Cell Body: In this electron micrograph, note some of the features you saw in ventral horn motor neurons with the light microscope, such as the large, pale nucleus, prominent nucleolus, Nissl bodies, dendrites and axon. Adjacent to the neuron, note myelinated axons of various sizes and also that there are no spaces between cell processes. All spaces are occupied either by the processes of neurons or glia or by capillaries (these capillaries are somewhat swollen here because the tissue was fixed by perfusion).
Node of Ranvier (longitudinal section): Remember that the node of Ranvier is actually a short segment of the axon that is bare at the junction between two Schwann cells, making "saltatory conduction" possible. Note the manner in which the myelin ends in each Schwann cell at the junction, by a "peeling off" of successive myelin layers, which come to lie against the axon as small cytoplasmic swellings.