Virtual Slide List


Access to the supplemental resources for this session is password-protected and restricted to University of Michigan students. If you are a University of Michigan student enrolled in a histology course at the University of Michigan, please click on the following link and use your Kerberos-password for access to download lecture handouts and the other resources.

Resources on M+Box

This collection was originally compiled by Kent Christensen, Ph.D., J. Matthew Velkey, Ph.D., Lloyd M. Stoolman, M.D., Laura Hessler, and Diedra Mosley-Brower. Currently, it is curated by Michael Hortsch, Ph.D. If you have questions or comments regarding the University of Michigan virtual slide collection, please contact Dr. Hortsch at hortsch at

Virtual Slide List for Histology Course

Search Description
Peripheral Nervous Tissue
Myelinated Nerve Fibers (longitudinal section): This image shows the typical appearance of a myelinated nerve, consisting of parallel bundles of axons (light areas) wrapped with sheaths of myelin (dark areas). As in the peripheral nervous system each Schwann cell myelinates only one axon, the discontinuous appearance of the axon labeled 5 in a Schwann cell is probably due to its curvature around the nucleus.
Schwann Cell with Myelinated Nerve Fiber (cross section): In this cross section of a myelinated nerve process, note the axon, containing microtubules and neurofilaments and bounded by a plasma membrane ("axolemma"). Outside the plasma membrane of the axon is the myelin sheath, which you will remember is composed of tightly wrapped plasma membranes of the Schwann cell. Also, note the nucleus and cytoplasmic organelles of the Schwann cell. Remember that the myelin is part of the Schwann cell, not of the axon.
Unmyelinated Nerve Fibers (cross section): The axons seen in this electron micrograph are all non-myelinated. They are embedded in grooves in the Schwann cell surface (in some cases there may be more than one axon per groove), with each Schwann cell thus supporting a considerable number of these small, unmyelinated axons. Although the axons are very close together, you will observe thin partitions of Schwann cell between them.
Multipolar Neurons (Celiac Ganglion): The celiac ganglia are autonomic ganglia. Note the large ganglion cells with somewhat eccentrically placed nuclei in several cells, a characteristic feature of autonomic ganglion cells.
Myelinated Nerve with Endoneurium and Perineurium (cross section): Between the axons you will see delicate connective tissue and an occasional fibroblast, which constitute the endoneurium. At the periphery of the fascicle, observe the perineurium, made up of several layers of flattened cells; it is a highly specialized layer that acts as a barrier and protects the nerve from the environment.
Neuromuscular Junction: This is a motor end plate. Note that the nerve axon loses its myelin sheath as it approaches the motor end plate and it terminates as a bulbous expansion in a trough of the muscle cell surface. The bulbous knob reveals numerous mitochondria and small synaptic vesicles, which contain cholinergic neurotransmitter substances. When these vesicles fuse the cell membrane of the axon bulb (the presynaptic membrane) and the content is released into the synaptic clefts to be taken up by the postsynaptic membrane (muscle cell membrane).
Neuromuscular Spindle: Note the two types of intrafusal muscle fibers, the nuclear bag fibers and nuclear chain fibers are enclosed by a delicate internal capsule. This arrangement serves as a muscle stretch receptor. The sensory nerve endings are activated by the stretching of the intrafusal fibers and the nerve impulse generated excites the somatic motor neurons in the spinal cord to evoke a reflex contraction of extrafusal fibers.
Respiratory System
Trachea, H&E, 40X
Palate, H&E, 20X (respiratory epithelium lining nasal cavity, stratified squamous lining oral cavity).
Fetal face, frontal section, H&E, 40X (nasal cavity, nasal mucosa, respiratory epithelium, olfactory epithelium).
Fetal face, horizontal section, H&E, 20X (nasal vestibule, nasal mucosa here is stratified squamous epithelium rather than respiratory epithelium).
Posterior nasal cavity, frontal sect., monkey, H&E, 40X
Fetal face, frontal section, H&E, 20X (nasal cavity, nasal mucosa, respiratory epithelium, olfactory epithelium).
Larynx, sagital section, H&E. 40X
Larynx, sagital section, H&E, 40X
Larynx, sagital section, H&E, 40X
Trachea and esophagus, H&E, 40X (in trachea: respiratory epithelium, hyaline cartilage, trachealis muscle, glands).
Trachea and esophagus, Masson stain, 20X (in trachea: respiratory epithelium, hyaline cartilage, trachealis muscle, glands).
Lung, H&E, 20X (bronchi, bronchioles, terminal bronchioles, respiratory bronchioles, alveolar ducts, alveolus, pulmonary arteries, bronchial arteries, pulmonary veins).
Lung, H&E, 20X (bronchi, bronchioles, terminal bronchioles, respiratory bronchioles, alveolar ducts, alveoli, greater alveolar cells = type II pneumocytes, macrophages = "dust cells", pulmonary arteries, bronchial arteries, pulmonary veins).
Lung, H&E, 40X
Lung, H&E, 40X
Lung, H&E, 20X (bronchi, bronchiole, terminal bronchioles, respiratory bronchioles, alveolar ducts, alveoli, pulmonary arteries, bronchial arteries, pulmonary veins).
Lung, H&E, 40X (bronchi, bronchiole, terminal bronchioles, respiratory bronchioles, alveolar ducts, alveoli, pulmonary arteries, bronchial arteries, pulmonary veins).
Alveoli: Study the organization of the alveoli. Note that individual alveoli border on each other and therefore share the wall, which is referred to as alveolar septum. Within the septum are found capillaries. Be able to recognize type I cells, type II cells and macrophages. Type II cells secrete surfactant and can be identified on the basis of their lamellar or multilamellar bodies, which cannot be seen well at this magnification. The type I cell lining the alveolus is also better identified at higher magnification (see EM #161).
Olfactory Epithelium: Two types of cells are shown in this EM micrograph; olfactory bipolar neurons with vesicles and cilia and supportive cells with microvilli on their apical surface. Note the absence of goblet cells in this neurosensory epithelium.
Respiratory/Pseudostratified Epithelium in the Trachea: Compare the morphology of the goblet cells with that of the other cells of the epithelium. Note that most of them are filled with secretory product (mucus) and that they do not have cilia. The line indicates the plane of section on the next wall chart #154.
Respiratory Epithelium (tangential section): The yellow colored area indicates the outline of one cell (see previous wall chart #153 for plane of section). Note the tops of the goblet cells protruding between the cilia.
Cilia: Review the fine structure of the cilium.
Bronchus: Note that the epithelium, although still pseudostratified, is not as tall as in the trachea (EM #153). Smooth muscle separates the lamina propria from the submucosa. Note the presence of a pulmonary artery and vein and that of a much smaller bronchial artery.
Alveolus (Blood-Air Barrier): Study the cellular and acellular components of the blood air barrier. Also study the type II pneumocyte in the upper right corner of this micrograph. Unfortunately, the lamellar bodies are not well preserved in this preparation.
Skin and Mammary Gland
Thin skin, stained for elastin, 20X (elastic fibers in the dermis).
Mesentery, H&E, 40X (Pacinian corpuscles)
Mesentary, Masson stain, 40X (Pacinian corpuscles)
Thick skin, sole of foot, H&E, 40X (thick skin, strata basale, spinosum, granulosum and corneum).
Thin skin, H&E, 40X
Thin skin, H&E, 40X
Thin skin, H&E, 40X (hair follicle).
Thin skin, H&E, 40X.
Thick skin, H&E, 40X (eccrine sweat gland; more and better in slide 107 40X).
Scalp, hair, H&E, 20X and 40X (hair follicle, external root sheath, sebaceous gland, arrector pili).
Fetal fingar, H&E, 20X (Pacinian corpuscle)
Fetal fingar Masson stain, 40X (Pacinian corpuscles)
Perianal region, Masson, 20X (apocrine sweat gland, myoepithelial cell).
Perianal region, H&E, 20X (at lower left, apocrine sweat gland, myoepithelial cell).
Axilla, subcutaneal region, Masson stain, 40X
Thick skin, sole of foot, 40X (thick skin, similar to slide 106).
Thick skin, sole of foot, 40X.
Active mammary gland, H&E, 40X (pregnant but not lactating, alveolus, myoepithelial cell).
Inactive mammary gland of nulliparous female, H&E, 20X (lobule, duct).